JCSG crystallization screen series
Joint Center for Structural Genomics formatted the JCSG Core Suite screens as a result of analyzing
over 500,000 high-throughput crystallization experiments, performed at the
JCSG. The 384 crystallization formulations were selected based on the highest
hit rates in initial screening.
The Wizard JCSG+™ 96 well block plate sparse matrix protein crystallization screen is designed for coarse screening of initial crystallization and solubility conditions for biological macromolecules. Researchers from the Joint Center for Structural Genomics (JCSG) formulated this screen with the goal of maximizing the coverage of the crystallization parameter space (buffers, salts, precipitants, and pH) and eliminating redundancy. The buffers give final solution pHs between 4.2 and 10.5. Precipitants include volatile reagents such as ethanol, high molecular weight polymers such as PEG-8000, and salts such as ammonium sulfate.
96-Well Block Plate formulations are 1.7 ml each in a 96 deep-well matrix block plate. All formulations are prepared with ASTM type-1 water and high-purity chemicals, 0.2 µm filtered in sterile packaging.
Wizard JCSG+ screen technical sheet
Wizard JCSG+ MSDS
JCSG Core Suite 1 screen technical sheet
JCSG Core Suite 2 screen technical sheet
JCSG Core Suite 3 screen technical sheet
JCSG Core Suite 4 screen technical sheet